Pharmacogenomics of Drug Transporters
Transporters play critical roles in the absorption, disposition, and elimination of numerous drugs that are in clinical use. Alterations in the expression and function of these transporters may significantly contribute to drug pharmacokinetics and the interindividual variability of drug response. Our research aims to unravel genetic and non-genetic factors that affect transporter expression and function. The long-term goal is to explore the impact of transporter Pharmacogenomics in disease cohorts already available at the Institute of Clinical Pharmacology.
Major Projects
- Molecular cell biology of drug transporters
- Functional characterization of drug transporters
- Pharmacogenomics of drug transporters
PD Dr. Anne Nies
phone: ++49-711-8101 3729
Selected References
- Nies AT, Koepsell H, Winter S, Burk O, Klein K, Kerb R, Zanger UM, Keppler D, Schwab M, Schaeffeler E (2009) Expression of organic cation transporters OCT1 (SLC22A1) and OCT3 (SCL22A3) is affected by genetic factors and cholestasis in human liver. Hepatology 50, 1227-1240.
- Nies AT, Schwab M, Keppler D (2008) Interplay of conjugating enzymes with OATP uptake transporters and ABCC/MRP efflux pumps in the elimination of drugs. Expert Opin Drug Metab Toxicol 4, 545-568.
- Nies AT, Herrmann E, Brom M, Keppler D (2008) Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1). Naunyn Schmiedebergs Arch Pharmacol 376, 449-461.
- Nies AT, Rius M, Keppler D (2007) Multidrug resistance proteins of the ABCC subfamily. In: You G, Morris M (eds.) Drug Transporters. John Wiley, Hoboken, NJ, pp. 263-318.
- Nies AT, Keppler D (2007) The apical conjugate efflux pump ABCC2 (MRP2). Pflügers Arch. 453, 643-659.
- Rius M, Thon WF, Keppler D, Nies AT (2005) Prostanoid transport by multidrug resistance protein 4 (MRP4/ABCC4) localized in tissues of human urogenital tract. J Urology 174, 409-414.
- Bronger H, König J, Kopplow K, Steiner HH, Ahmadi R, Herold-Mende C, Keppler D, Nies AT (2005) ABCC drug efflux pumps and OATP uptake transporters in human gliomas and the blood-tumor barrier. Cancer Res 65, 11419-11428.
- Nies AT, Jedlitschky G, König J, Herold-Mende C, Steiner HH, Schmitt HP, Keppler D (2004) Expression and immunolocalization of the multidrug resistance proteins, MRP1-MRP6 (ABCC1-ABCC6), in human brain. Neuroscience 129, 349-360.
- Michalski C, Cui Y, Nies AT, Nuessler AK, Neuhaus P, Zanger UM, Klein K, Eichelbaum M, Keppler D, König J (2002) A naturally occurring mutation in the SLC21A6 gene causing impaired membrane localization of the hepatocyte uptake transporter. J Biol Chem 277, 43058-43063.
- Nies AT, König J, Pfannschmidt M, Klar E, Hofmann WJ, Keppler D (2001) Expression of the multidrug resistance proteins MRP2 and MRP3 in human hepatocellular carcinoma. Int J Cancer94, 492-499.
Curriculum Vitae Anne Nies, PhD
Affiliation
Head Membrane Transport Proteins
Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology
Auerbachstrasse 112
70376 Stuttgart Germany
phone: ++49-711-8101 3729
fax: ++49-711-859295
email: anne.nies@ikp-stuttgart.de
Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology
Auerbachstrasse 112
70376 Stuttgart Germany
phone: ++49-711-8101 3729
fax: ++49-711-859295
email: anne.nies@ikp-stuttgart.de
Education and Professional Career
| 1984-1990 | Studies of Biology, University of Düsseldorf, Germany |
| 1990 | Diploma in Biology, University of Düsseldorf |
| 1990-1993 | PhD thesis in Biology, University of Düsseldorf |
| 1993 | Dr. rer. nat. (Ph.D.) in Biology, University of Düsseldorf, on transport and metabolism of urate in the hepatopancreas of the lobster |
| 1994-1996 | Postdoctoral Research Fellow of the Deutsche Forschungsgemeinschaft at Tufts University, Department of Physiology (Boston, MA, USA), on the biochemistry und molecular biology of P-glycoprotein and other hepatic transport systems |
| 1996-2007 |
Senior Research Scientist at the German Cancer Research Center, Division of Tumor Biochemistry, Heidelberg, Germany. Research area: Molecular cell biology of ATP-dependent efflux pumps and uptake transporters in the plasma membrane |
| 2004 | Habilitation and venia legendi in Biochemistry, University of Heidelberg Medical School |
| Since 2007 | Senior Group Leader and Head of Membrane Transport Proteins, Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany |
| Since 2008 | Venia legendi in Biochemical Pharmacology, University of Tübingen Medical School, Germany |