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Imaging

Staff
Staff
  • Silvia Hübner
    laboratory technician
  • Kerstin Willecke
    laboratory technician
Infrastructure and Functions
The Imaging core facility offers a broad range of “state-of-the-art” light and fluorescence microscopy (in biosafety levels 1 and 2 labs) as well as flow cytometry (biosafety level 1).
The imaging core facility provides access to a confocal laser scanning microscope, several fluorescence and wide field microscopes, a laser-capture microscope, a digital slide scanner, as well as a flow cytometer including a sorter module. This equipment allows high resolution imaging of cellular and sub-cellular structures on the one hand and high throughput analyses on the other. We perform, for instance, imaging and imaging analyses from tissue sections or isolated cells, 3D reconstructions, protein-protein interaction studies, fluorescence in situ hybridization, cell culture life imaging, and high throughput fluorescence imaging.  
As part of the European Union-funded PREDECT consortium  and the BMBF-funded initiative on predicting effective treatment of cancer PREDICT the imaging facility provides major support for the different project partners.

In detail, the imaging facility is equipped with:

  • High performance microscope equipped with high resolution color digital camera (Leica DM4000), motorized object table    (x-y), discussion-bridge, serial imaging module, and a comprehensive software package for quantitative analyses and image processing

  • High resolution digital scanner (Leica SCN400) for digitizing whole microscopic slides. A high speed dynamic focus limits scanning time and risk of failed scans

  • Several small light microscopes (Olympus and Zeiss)

  • Zeiss PALM-MicroBeam laser-micro dissection based on the Axiovert 200M for isolation of single cells or cellular areas from paraffin embedded sections or cryosections for preparation of DNA, RNA, or protein

  • Olympus inverse small wide field and fluorescence microscope especially for the biosafety 2 lab equipped with diverse filters for detection of fluorescent proteins. High quality phase contrast objectives allow live-cell imaging. The system is equipped with a high resolution camera and analysis software

  • High performance fluorescence microscope especially for FISH (fluorescence in situ hybridization) analyses (Leica DMRA). Equipped with 7 filters covering wavelengths from UV to dark red, a high-resolution digital camera as well as a FISH analysis software (IKAROS/ISIS)

  • Confocal laser scanning microsope (Leica DMIRBE) equipped with a fluorescence prism allowing high flexibility in choosing and combining fluorochromes in the range between green and dark red. The system is combined with analysis software for quantification of fluorescence intensities and 3D imaging

  • InCell Analyzer (GE Healthcare): fluorescence microscope for automated high content cellular and sub-cellular imaging. Equipped with 4 fluorescence filters and wide field as well as a life chamber maintaining 37°C and allowing live cell imaging. A fully automated liquid-handling module allows for dispensing or aspirating a reagent during imaging. A detailed software package allows analysis of more than 50 different applications for quantification of cellular and subcellular structures and processes

  • Flow cytometer (FACS-Calibur, Becton Dickinson) for detection of 3 different fluorochromes, cell size and cellular granulation. A sorter module allows isolating a population with defined characteristics

  • Besides microscopy and flow cytometry the imaging core facility comprises also microtomes and auto stainer for slide preparation and high-throughput slide staining

  • Imaging analysis software (Definiens Tissue Studio) for automated determination of morphology and expression profiles per individual cell or cellular compartment